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Following RO, bEnd.3 cells were washed twice with pre-warmed Krebs-HEPES buffer [composed of (in mmol/L) 99 NaCl, 4.7 KCl, 1.2 MgSO 4 , 1.0 KH 2 PO 4 , 19.6 NaHCO 3 , 11.2 Glucose 20 Na-HEPES and 2.5 CaCl 2 , pH = 7.4] prior to incubating with the MTS solution for 1 h at 37 C (5% CO 2 )

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