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Von Frey test We assessed mechanical pain sensitivity by observing the paw withdrawal reaction to von Frey filament stimulation

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Levels of evidence and grades of recommendation The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) system (Table 1) was applied to grade the evidence and recommendations

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Consistently, SureQuantbased targeted mass spectrometry using two unique CEST2.2 peptides (LSTGTIEGR and HGEDFAYAFGTNR) revealed that our transgene could also restore CEST2.2 protein levels when introduced in gas1(fc21) mutants (Fig 1L and Appendix Table S2)